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Archive for the 'Uncategorized' Category

Risk Assessment of Cell Culture

Wednesday, June 18th, 2008

The main aim of risk assessment is to prevent injury, protect property and avoid harm to individuals and the environment. The performance of risk assessment is a legal requirement under the Health and Safety at Work Act, UK. There are other EC directives covering Health and Safety at Work, you can visit the European Agency […]

Subculture of Adherent Cell Lines

Tuesday, June 17th, 2008

Adherent cell lines will grow in vitro until they have covered the surface area available or the medium is depleted of nutrients. At this point the cell lines should be sub-cultured in order to prevent the culture dying. To subculture the cells they need to be brought into suspension. The degree of adhesion varies from […]

Resuscitation of Frozen Cell Lines

Thursday, June 12th, 2008

Many cultures obtained from a culture collection, such as ECACC, will arrive frozen and in order to use them the cells must be thawed and put into culture. It is vital to thaw cells correctly in order to maintain the viability of the culture and enable the culture to recover more quickly. Some cryoprotectants, such […]

Aseptic Technique and Good Cell Culture Practice

Wednesday, June 11th, 2008

AimTo ensure all cell culture procedures are performed to a standard that will prevent contamination from bacteria, fungi and mycoplasma and cross contamination with other cell lines.
Materials

Chloros / Presept solution (2.5g/l)
1% formaldehyde based disinfectant e.g.Virkon,Tegador
70% ethanol in water (Prod. No. R8382)

Equipment

Personal protective equipment (sterile gloves, laboratory coat, safety visor)
Microbiological […]

The “Do’s and Don’ts” of Cell Culture

Friday, June 6th, 2008

Since it was established in 1981, the Cell Culture Facility has made it easier, more economical and more effective to use mammalian cell cultures in research at Fox Chase. Cell culture procedures provide the cellular materials for many of the molecular methods used to study normal and cancer cell […]

Silver Jewelry Wear Rules

Thursday, June 5th, 2008

Silver jewelry is a special thing in your daily life. You should find the best way to wear it. Maybe you are very puzzled how to choose a silver jewelry for your cloth. Now there are some rules can help you.
1. Professional clothes should come together with simple\ fine style silver necklace.
2. Leisure clothes should […]

How To Choose The Right Bracelet

Monday, June 2nd, 2008

In this article we explain how to choose the right bracelet. Your bracelet could be made in silver or gold, it could be a simple bracelet or be studded with stones and diamonds. Make sure that you know how to evaluate a good bracelet.
Step One
Basic Design: The first thing that you should consider is the […]

Six Tips To Help You Pass Exam 70-291

Wednesday, May 14th, 2008

In this article, we won’t go through each objective but rather focus on six of the most important things to know to be prepared for Exam 70-291. But first, let’s take a minute to determine whether you need to tackle the exam at all.
Do you need to take this exam?
Exam 70-291 is intended to verify […]



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